Ação interdisciplinar de promoção à saúde no programa escola da família: relato de experiência de residentes do programa multidisciplinar em saúde da família / Interdisciplinary action on health promotion in the school of family program: experience report of residents in the multidisciplinary program in family health / Acción interdisciplinar de promoción a la salud en el programa escuela de la familia: relato de experiencia de estudiantes posgraduados del programa multidisciplinar en salud de la familia

Relato da experiência vivenciada por alunos da Residência Multiprofissional em Saúde da Família a partir da participação no Programa Escola da Família. Método: Relato que mostra os resultados da prática de propostas interdisciplinares para os participantes do Programa Escola da Família em uma região periférica de uma cidade do interior paulista. Resultado: A participação de uma equipe multidisciplinar teve impacto positivo no programa, uma vez que a diversidade de profissões permitiu uma abordagem mais ampla. A participação no programa, além de proporcionar uma experiência intersetorial, nos levou a articular ações, unir os profissionais da residência e os profissionais da educação e suas diferentes experiências e conhecimentos. Conclusão: A participação de pós-graduandos multiprofissionais em Saúde da Família gera interação com a comunidade, aumentando sua relação com a unidade de saúde, aproximando crianças e adolescentes e promovendo a autonomia da atenção à saúde em geral. A prática interdisciplinar é necessária para enriquecer as atividades propostas.(AU)

Report the experience lived by multiprofessional postgraduated students in Family Health from the participation in the School's Family Program. Method: A report that shows results from the practice of interdisciplinary proposals for participants of the School's Family Program in a peripheral region in a countryside city of São Paulo. Result: The participation of a multidisciplinary team had a positive impact on the program, since the diversity of professions allowed a broad approach. The practice in the program besides providing an intersectoral experience brought us to link actions, uniting multiprofessional students and educational professionals and its different experiences and knowledge. Conclusion: The participation of the multiprofessional team in Family Health generates interaction with the community, increasing their link with the health unit, approaching children and teenagers and promotes the autonomy of health care as a whole. Interdisciplinary practice is necessary to highlight the proposed activities.

Informe la experiencia vivida por estudiantes posgraduados multiprofesionales en Salud de la Familia a partir de la participación en el Programa de la Escuela de la Familia. Método: Un informe que muestra los resultados de la práctica de propuestas interdisciplinarias para los participantes del Programa de la Escuela de la Familia en una región periférica de una ciudad del interior de São Paulo. Resultado: La participación de un equipo multidisciplinario tuvo un impacto positivo en el programa, ya que la diversidad de profesiones permitió un enfoque amplio. La práctica en el programa, además de proporcionar una experiencia intersectorial, nos llevó a vincular acciones, unir a estudiantes de postgrado multiprofesionales y profesionales de la educación y sus diferentes experiencias y conocimientos. Conclusión: La participación de los estudiantes de postgrado multiprofesional en Salud Familiar genera interacción con la comunidad, incrementando su vinculación con la unidad de salud, acercándose a niños y adolescentes y promoviendo la autonomía de la atención de la salud en general. La práctica interdisciplinaria es necesaria para enriquecer las actividades propuestas.(AU)

A century of research: what have we learned about the interaction of Trypanosoma cruzi with host cells?

Since the discovery of Trypanosoma cruzi and the brilliant description of the then-referred to "new tripanosomiasis" by Carlos Chagas 100 years ago, a great deal of scientific effort and curiosity has been devoted to understanding how this parasite invades and colonises mammalian host cells. This is a key step in the survival of the parasite within the vertebrate host, and although much has been learned over this century, differences in strains or isolates used by different laboratories may have led to conclusions that are not as universal as originally interpreted. Molecular genotyping of the CL-Brener clone confirmed a genetic heterogeneity in the parasite that had been detected previously by other techniques, including zymodeme or schizodeme (kDNA) analysis. T. cruzi can be grouped into at least two major phylogenetic lineages: T. cruzi I, mostly associated with the sylvatic cycle and T. cruzi II, linked to human disease; however, a third lineage, T. cruziIII, has also been proposed. Hybrid isolates, such as the CL-Brener clone, which was chosen for sequencing the genome of the parasite (Elias et al. 2005, El Sayed et al. 2005a), have also been identified. The parasite must be able to invade cells in the mammalian host, and many studies have implicated the flagellated trypomastigotes as the main actor in this process. Several surface components of parasites and some of the host cell receptors with which they interact have been described. Herein, we have attempted to identify milestones in the history of understanding T. cruzi- host cell interactions. Different infective forms of T. cruzi have displayed unexpected requirements for the parasite to attach to the host cell, enter it, and translocate between the parasitophorous vacuole to its final cytoplasmic destination. It is noteworthy that some of the mechanisms originally proposed to be broad in function turned out not to be universal, and multiple interactions involving different...

Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi

Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53 per cent of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C16:1 and C18:1 when compared to the total fatty acid pool isolated from the parasites.